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Apr
26


Crano Memorial Lecture of Akron ACS
April 26, 2019 5:30 PM
2:30 PM: 111 Mary Gladwin Hall, U. Akron; 7:00 PM: The Overlook Grill, 1519 Overlook Rd., Kent, OH 44240    Location
Host: ACS

Crano Award Lecture Friday, APR 26, 2019 Professor Bruce Armitage Carnegie Mellon University Afternoon Program 2:30 PM, 111 Mary Gladwin Hall, University of Akron Evening Program The Overlook 1519 Overlook Rd, Kent OH 44240 5:30 PM Networking 6:30 PM Dinner 7:00 PM Lecture (both lectures are free to all) Dinner Reservations: RSVP to cmkausch@hotmail.com $25 for members / public and $10 for students Please specify: 1) Peach Chicken, 2) Ribeye or 3) Eggplant parmesan
Afternoon Lecture at 2:30 PM, 111 Mary Gladwin Hall, University of Akron "Fluorescence Imaging Reagents Based on RNA Aptamers, Synthetic Polymers and Fluorogenic Cyanine Dyes" The seminar will describe two fluorescent labeling technologies under development in our laboratory. The first technology relies on an RNA aptamer that binds to and activates fluorogenic cyanine dyes, giving either blue or red fluorescence, depending on the structure of the dye. The utility of this promiscuous aptamer is demonstrated by experiments in which it is fused to a separate RNA that recognizes a cell-surface receptor protein, leading to fluorescent labeling. Internalized and cell-surface receptor can be distinguished temporally based on two successive labeling steps in which either blue or red fluorogen is applied to the sample. The second part of the seminar describes recent work toward improved brightness from fluorescent labels. Whereas most fluorescent reagents (e.g. antibodies) are labeled with a few dyes, we have developed a hybrid material consisting of a polymeric scaffold from which is grafted double-helical DNA that acts as a host for fluorescent intercalating dyes. The resulting nanotags host >1000 dyes with minimal quenching and can be attached to antibodies for use in fluorescence microscopy, flow cytometry and dot blotting applications.
Evening Lecture at Overlook Grill, Kent: DNA Nanotechnology:When Encoding the Genome Just Isn’t Enough The core of the Watson and Crick double-helical model for the structure of DNA is the complementary base pairing of purine and pyrimidine units. This pairing also provides the foundation for the burgeoning field of DNA nanotechnology, where complementary DNA strands associate with one another to form complex 2- and 3-D shapes. This field was initially concerned with the synthesis of structured materials but has evolved to one that is increasingly driven by applications. Contributions from the Armitage lab to the use of DNA nanostructures in the field of biological imaging will be highlighted. Our work is illustrative of collaborations among scientists in two multidisciplinary research centers at Carnegie Mellon University: the Center for Nucleic Acids Science and Technology (www.cmu.edu/cnast) and the Molecular Biosensor and Imaging Center (http://pathways.mbic.cmu.edu).
Biography: Professor Armitage is the Co-Director at the Carnegie Mellon Center for Nucleic Acids Science and Technology of Chemistry.
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